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Kansas School Naturalist


KSN - Vol. 53, No. 2 - front coverVolume 53, Number 2 - December 2006

Life History of Kansas Freshwater Mussels

by Brian K. Obermeyer, Edwin J. Miller and M. Christopher Barnhart
Photos by M. Christopher Barnhart

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ABOUT THIS ISSUE

ISSN: 0022-877X
Published by EMPORIA STATE UNIVERSITY
Editor: JOHN RICHARD SCHROCK
Editorial Committee: TOM EDDY, BILL JENSEN, R. BRENT THOMAS, ERIC YANG
Editors Emeritus: ROBERT BOLES, ROBERT F. CLARKE
Circulation and Mailing: ROGER FERGUSON
Circulation (this issue): 10,000 Press Composition: John Decker
Press Run: 15,000
Printed by: ESU Printing Services
Online edition by: TERRI WEAST

The Kansas School Naturalist is sent free of charge and upon request to teachers, school administrators, public and school librarians, youth leaders, conservationists, and others interested in natural history and nature education. Back issues are sent for one dollar photocopy and postage/handling charge per issue. A back issue list is sent free upon request. The Kansas School Naturalist is sent free upon request by media mail to all U.S. zipcodes, first class to Mexico and Canada, and surface mail overseas. Overseas subscribers who wish to receive it by airmail should remit US $5.00 per four issues airmail and handling. The Kansas School Naturalist is published by Emporia State University, Emporia, Kansas. Postage paid at Emporia, Kansas. Address all correspondence to: Editor, Kansas School Naturalist, Department of Biological Sciences, Box 4050, Emporia State University, Emporia, KS 66801-5087. Opinions and perspectives expressed are those of the authors and/or editor and do not reflect the official position or endorsement of ESU. Some issues can be viewed online at: www.emporia.edu/ksn/ The Kansas School Naturalist is listed in Ulrich’s International Periodicals Directory, indexed in Wildlife Review/Fisheries Review, and appropriate issues are indexed in the Zoological Record.


Front cover: Verdigris River shell midden.


Authors:

Picture - Brian Obermeyer  Brian Obermeyer is the the Flint Hills Initiative Director for the Kansas Chapter of The Nature Conservancy, a nonprofit organization that strives to protect Earth’s biodiversity. Obermeyer leads a community-based conservation program dedicated to preserving the Flint Hills, the last large expanse of tallgrass prairie, through a collaborative approach with ranchers, landowners and other stakeholders.
 Picture - Chris Barnhart  Chris Barnhart is Professor of Biology at Missouri State University. He received his M.S. degree from the University of Kansas at Lawrence and Ph.D. from the University of California, Los Angeles. Barnhart studies the life history and reproductive biology of unionid mussels and has authored more than 50 publications and reports on the physiology, ecology, and husbandry of mollusks and other invertebrates.
 Picture - Edwin J. Miller  Edwin J. Miller has been employed by Kansas Department of Wildlife and Parks since 1989. Miller’s position is Threatened and Endangered Species Program Coordinator. He is intrigued with the diversity and function of the native mussels in Kansas and enjoys getting others interested in this important faunal group. He coordinates a Kansas Pearly Mussel Workshop each summer and is currently preparing a pocket guide to the mussels of Kansas. His formal education includes a M.S. in Wildlife Science from Virginia Tech and a B.S. in Animal Ecology from Iowa State University. He lives with his wife and
two daughters in Independence, KS.


Life History of Kansas Freshwater Mussels

by Brian K. Obermeyer, Edwin J. Miller and M. Christopher Barnhart
Photos by M. Christopher Barnhart

The world’s greatest diversity of freshwater mussels (Unionoida) is concentrated in North America, with approximately 300 species and subspecies (31).

MANY THREATENED
This rich historical mussel fauna is in serious jeopardy. Freshwater mussels are considered the most imperiled group of animals in North America (1). The U.S. Fish and Wildlife Service lists 70 species as endangered or threatened, and 17 more are presently candidates for listing. At least 36 species are believed to be extinct (22). Kansas mussels have undergone a similar decline. Of the 46 species known
to have occurred in the state, seven are now state-listed as endangered, four as threatened, 11 as SINC (species in need of conservation), and at least five species are probably extirpated from the state (8, 14, 25).

Table 1. Kansas mussel species considered to be at risk (E - endangered, T - threatened, SINC - species in need of conservation, X - extirpated, R - rare but not listed, U - under review)

 

Kansas status

Margaritiferidae  
Cumberlandia monodonta - spectaclecase X
Unionidae  
Actinonaias ligamentina - mucket E
Alasmidonta marginata - elktoe E
Alasmidonta viridis - slippershell mussel X
Anodonta suborbiculata - flat floater E
Anodontoides ferussacianus - cylindrical papershell SINC
Arcidens confragosus - rock pocketbook T
Cyclonaias tuberculata - purple wartyback U
Cyprogenia aberti - western fanshell E
Ellipsaria lineolata - butterfly T
Elliptio dilatata - spike SINC
Epioblasma triquetra - snuffbox X
Fusconaia flava - Wabash pigtoe SINC
Lampsilis siliquoidea - fatmucket SINC
Lampsilis rafinesqueana - Neosho mucket E
Lampsilis teres - yellow sandshell SINC
Lasmigona costata - flutedshell T
Ligumia recta - black sandshell R
Megalonaias nervosa - washboard SINC
Obovaria olivaria - hickorynut X
Pleurobema sintoxia - round pigtoe SINC
Ptychobranchus occidentalis - Ouachita kidneyshell T
Quadrula cylindrica - rabbitsfoot E
Quadrula fragosa - winged mapleleaf X
Quadrula nodulata - wartyback SINC
Strophitus undulatus - creeper SINC
Truncilla donaciformis - fawnsfoot SINC
Truncilla truncata - deertoe SINC
Venustaconcha ellipsiformis - ellipse E


LIFE STAGES

KSN - Vol. 53, No. 2 - figure 1
Figure 1: Four basic life stages of freshwater mussels.

 


 

The life history of freshwater unionid mussels is fascinating and complex, and consists of four basic life stages: reproductive, larval or parasitic, juvenile, and adult (Figure 1). Most mussels are dioecious (having separate sexes). Males release sperm into the water, and the sperm are filtered from the water by the female. Fertilized eggs are brooded within the female’s marsupial gills, which contain hollow spaces for this purpose. Fecundity varies among species, ranging from 75,000 to 3,000,000 larvae per female (13, 29). Mussel larvae, called glochidia, may be released soon after they are mature, or may be retained in the gills for several months or until the next season (26). Species that release glochidia soon after they are mature are called short-term brooders (tachytictic), whereas species that retain their glochidia for extended periods of time are referred to as long-term brooders (bradytictic). Tachytictic species generally spawn in the spring or early summer, whereas bradytictic species usually spawn during late summer or fall months.

 


 

PARASITIC STAGE COMMON

Figure 2. A fish usually serves as the host to mussel glochida. Drawing courtesy of Scott Faiman.

Figure 2. A fish usually serves as the host to mussel glochida. Drawing courtesy of Scott Faiman.
Juvenile mussels Close-up of glochidia attached to gill filaments of host fish Host fish Host fish Gravid female mussel releasing glochidia

Glochidia must briefly parasitize a vertebrate host (usually a fish) to complete their development 1 (Figure 2). The primary function of larval parasitism on fish appears to be transport to upstream habitats (30). Larvae attached to fish may be carried upstream, whereas adult mussels are not very mobile, and unattached larvae can only drift downstream.

Glochidia must come in contact with a fish host soon after leaving the female mussel. Only a small percentage of glochidia actually make contact with a suitable host. Upon contact with a gill filament, a fin, or the epithelium of a fish, a glochidium clamps on. However, glochidia cannot discriminate between suitable and non-suitable hosts, and may snap shut in response to just about any stimulus. If the glochidium attaches to an unsuitable host, it will be rejected and sloughed off. On a suitable host, the tissue encapsulates the glochidium by migration of epithelial cells (Figure 3). In most species the encapsulation period lasts from 2 to 3 weeks, although it can range from 6 days to 7 months depending on species and temperature (19).

1 Only one North American species, the green floater (Lasmigona subviridis), is positively known to bypass the parasitic life phase (2, 21).

KSN - Vol. 53, No. 2 - Figure 3. Gill filament tissue

Figure 3. Gill filament tissue encapsulates glochidia; (insert) released glochidia before attaching to a suitable host.

Mussels have evolved some fascinating reproductive adaptations to increase the chances that glochidia will make contact with a suitable host. Females of the genus Lampsilis extend a pair of mantle flaps (actually an extension of the inner lobe of the mantle edge; 20) that, from a side angle, remarkably resembles a small fish (Figure 4). Each mantle flap, in addition to its fish-like shape, has pigmentation that resembles an eyespot as well as a fish’s lateral line. Muscular contractions of the mantle flaps create an undulating or“swimming” motion that apparently acts as a lure to attract potential fish hosts (6, 18). If a fish strikes at the lure, it ruptures the swollen marsupial gills, which extend between the two mantle flaps, releasing a cloud of glochidia.

Figure 4. Mantle flaps of this mussel resemble a small fish.

Figure 4. Mantle flaps of this mussel resemble a small fish.

MUSSELS THAT BAIT FISH

A number of species are what might be referred to as “bait fishermen,” releasing masses of eggs called conglutinates to be fed upon by host fish. When the conglutinate is bitten, glochidia are dislodged from the eggs and attach to the host’s gills. Many riverine mussel species use this strategy. Conglutinates of some species, such as fanshells, consist mostly of sterile eggs that are often brightly colored and relatively opaque. They appear to serve to make the conglutinate more visible and perhaps more palatable to the host fish.

The female western fanshell releases a particularly long conglutinate or lure (as much as 8 cm in length) to attract its host fish (Figure 7). Each conglutinate consists of approximately 30,000 eggs (3, 4). Only the eggs along the periphery of the conglutinate are fertilized (~15–20 percent of the total). The marsupial gills of this species are coiled (9, 10, 11, 12) (Figure 6), which function to accommodate the long, worm-like conglutinates (3, 12, 27).

Figure 5. Kidneyshell, conglutinates, and orangethroat darter, a probable host.

Figure 5. Kidneyshell, conglutinates, and orangethroat darter, a probable host.

Figure 6. Marsupial gills in this species are coiled to produce the long lure.

Figure 6. Marsupial gills in this species are coiled to produce the long lure.

The female Ouachita kidneyshell releases glochidia packets that strikingly resemble larval fish (Figure 5). Each packet contains 200-plus glochidia housed inside a membranous sheath, measuring 1 to 1.5 cm in length (6). Glochidia packets are readily taken as food by darters, which, during the process of consumption, infect themselves with glochidia.

While many species attach primarily to the gills of the host, others attach to fins or the epithelia of fish. The creeper mussel utilizes the “leghold trap” strategy to make contact with a host. Conglutinates are adhesive and stick to the substratum. Each conglutinate consists of roughly 1–15 eggs, usually arranged in single-file. The glochidia “hatch” shortly after release of the conglutinate, but remain tethered to it by short larval threads (similar to the chain of a leg-hold trap (Figure 8).

Figure 7. Western fanshell releasing conglutinates; (insert) conglutinates of Western fanshell come in several colors.
Figure 7. Western fanshell releasing conglutinates; (insert) conglutinates of Western fanshell come in several colors. Figure 7. Western fanshell releasing
conglutinates; (insert) conglutinates of Western fanshell come in several colors.

The glochidia are large and have prominent hooks, and they attach readily to the fins of benthic fishes. The larval thread is quite strong, and the whole conglutinate may be pulled along if even one glochidium attaches to a fin. At that point, it becomes the “tar baby” strategy as more glochidia may attach to the fin.

While attached to its fish host, the glochidium undergoes a metamorphosis, transforming from the glochidial stage to a juvenile. Some species grow during this period but in most species there is no noticeable change in size. The changes in anatomy are profound, however, with most of the organ systems developing for the first time. Following metamorphosis, the juvenile mussel will excyst, drop from the fish, and as it matures, eventually take up life as a sedentary filter feeder (Figure 9). The percentage of glochidia that reach this stage is extremely small.

Figure 8. Glochidia “hatch” from the conglutinate but remain briefly tethered by larval threads.

Figure 8. Glochidia “hatch” from the conglutinate but remain briefly tethered by larval threads.

The juvenile or post-parasitic stage represents the period from metamorphosis to when a young mussel produces gametes, which usually occurs from two to six years of age for most species in Kansas. This stage, especially during the first few months, is thought to be a vulnerable link in the life cycle of freshwater mussels (15, 23, 28). Specific ecological requirements of juvenile mussels remain unknown for most species. Attempts to raise juveniles have only recently yielded acceptable results (5, 16, 17, 23).

The adult life stage is typically what most people envision when they think about freshwater mussels (back cover). Consequently, past mussel research has largely focused on this life stage. Fortunately, researchers have recently begun to address the entire life cycle of freshwater mussels. Nonetheless, emphasis on the adult life stage is appropriate for certain aspects of mussel research, such as distributional assessments, especially because many species are long lived. For example, one washboard (Megalonaias nervosa) from the Neosho River was estimated to be nearly a century in age (based on an acetate peel count of approximately 95 annuli) (24). Interestingly, gonad samples from this specimen yielded spermatozoa. This observation is not particularly surprising since Bauer (7) found that the reproductive output of M. margaritifera, another longlived freshwater mussel that commonly exceeds 100 years in age, does not decline significantly with age. The continued reproductive fitness of aged mussels may have important management implications (24).

Figure 9. Juvenile mussels are smaller than the head of a pin.

Figure 9. Juvenile mussels are smaller than the head of a pin.

REFERENCES

  1. Allan, J.D., and A.S. Flecker. 1993. Biodiversity conservation in running waters. BioScience 43:32–43.
  2. Barfield, M.L., and G.T. Watters. 1998. Non-parasitic life cycle of the green floater, Lasmigona subviridis (Conrad 1835). Triannual Unionid Report 16:22
  3. Barnhart, M.C. 1997a. Sterile eggs in unionid mussels and their roles in conglutinate function. Triannual Unionid Report 11:25
  4. Barnhart, M.C. 1997b. Conglutinates and fish hosts of the western fanshell (Cyprogenia aberti). Triannual Unionid Report 12:2
  5. Barnhart, M.C. 2006. A compact system for rearing juvenile freshwater mussels. Aquaculture 254:227–233
  6. Barnhart, M.C., and A. Roberts. 1997. Reproduction and fish hosts of unionids from the Ozark Uplifts. Pages 15-20 in K.S. Cummings, A.C. Buchanan, C.A. Mayer, and T.J. Naimo (editors). Conservation and management of freshwater mussels II: Initiatives for the future. Proceedings of a UMRCC Symposium, 16-18 October 1995, St. Louis, MO. Upper Mississippi River Conservation Committee, Rock Island, IL.
  7. Bauer, G. 1987. Reproductive strategy of the freshwater pearl mussel, Margaritifera margaritifera. Journal of Animal Ecology 56:691-704.
  8. Bleam, D.E., C.H. Cope, K.J. Couch, and D.A. Distler. 1998. The winged mapleleaf, Quadrula fragosa (Conrad 1835) in Kansas. Transactions of the Kansas Academy of Science 101(1- 2):35-38.
  9. Call, R.E. 1885. Description of a new species of Unio from Kansas. Bulletin of the Washburn College Laboratory of Natural History 1:48-49.
  10. Call, R.E. 1887. Sixth contribution to a knowledge of fresh-water mollusca of Kansas. Bulletin of the Washburn College Laboratory of Natural History 2(8):11-25.
  11. Call, R.E. 1887. Note on the ctenidium of Unio aberti Conrad. American Naturalist 21:857-860.
  12. Chamberlain, T.K. 1934. The glochidial conglutinates of the Arkansas fanshell, Cyprogenia aberti (Conrad). Biological Bulletin 66:55-
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  14. Couch, K.J. 1997. An illustrated guide to the unionid mussels of Kansas. Privately published by Karen J. Couch. 123 pp.
  15. Dimock, R.V., Jr., and A.H. Wright. 1993. Sensitivity of juvenile freshwater mussels to hypoxic, thermal and acid stress. The Journal of the Elisha Mitchell Scientific Society 109:183-192.
  16. Gatenby, C.M., R.J. Neves, and B.C. Parker. 1996. Influence of sediment and algal food on cultured juvenile freshwater mussels. Journal of the North American Benthological Society 15(4):597-609.
  17. Gatenby, C.M., B.C. Parker, and R.J. Neves. 1997. Growth and survival of juvenile rainbow mussels, Villosa iris (Lea, 1829) (Bivalvia: Unionidae), reared on algal diets and sediment. American Malacological Bulletin 14(1):57-66.
  18. Gordon, M.E., and J.B. Layzer. 1989. Mussels (Bivalvia: Unionoidea) of the Cumberland River: review of life histories and ecological relationships. U.S. Fish and Wildlife Service Biological Report 89(15). 99 pp.
  19. Howard, A.D. 1915. Some exceptional cases of breeding among the Unionidae. The Nautilus 29:4– 11.
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  21. Lellis, W.A., and T.L. King. 1998. Release of metamorphosed juveniles by the green floater, Lasmigona subviridis. Triannual Unionid Report 16:23.
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  24. Obermeyer, B.K. 1997. Survey of freshwater mussels in deep-water habitats in the Neosho River, KS. Unpublished report to the Kansas Department of Wildlife and Parks, Pratt, KS. 23 pp.
  25. Obermeyer, B.K., D.R. Edds, C.W. Prophet, and E.J. Miller. 1997. Freshwater mussels (Bivalvia: Unionidae) in the Verdigris, Neosho, and Spring river basins of Kansas and Missouri, with emphasis on species of concern. American Malacological
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  28. Sparks, B.L., and D.L. Strayer. 1998. Effects of low dissolved oxygen on juvenile Elliptio complanata (Bivalvia: Unionidae). Journal of the North American Benthological Society 17(1):129–134.
  29. Surber, T. 1912. Identification of the glochidia of fresh-water mussels. U.S. Bureau of Fisheries Document 771, 10 p.
  30. Surber, T. 1913. Notes on the natural hosts of fresh-water mussels. Bulletin of the U.S. Bureau of Fisheries 32: 101- 116.
  31. Turgeon, D. D., J.F. Quinn, Jr., A. E. Bogan, E. V. Coan, F.G. Hochberg, W.G. Lyons, P.M. Mikkelsen, R.J. Neves, C. F. E. Roper, G. Rosenberg, B. Roth, A. Scheltema, F. G. Thompson, M. Vecchione, and J. D. Williams. 1998. Common and scientific names of aquatic invertebrates from the United States and Canada: mollusks. Second edition. American Fisheries Society Special Publication 26. Bethesda, MD.

KSN - Vol. 53, No. 2 - back cover


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