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Volume 50, Number 1, December 2003:
A Toxicology Primer for Student Inquiry: Biological Smoke Detectors

Text-only version

ISSUE HOME PAGE


ABOUT THIS ISSUE
- about KSN
- about the author
- acknowledgements

IN THIS ISSUE
- disclaimer, objectives
- "biological smoke detectors"
- purpose of invertebrate toxicity testing
- lethal and sublethal effects
- some wormy ideas for toxicity testing
- sublethal chemical effects in lumbriculus
- selecting the chemical(s)
- safety
- exposure methods
- preliminary experiments and concentration range-finding
- final stages of toxicity testing
- typical equipment and supplies
- other organisms, other ideas
- obtaining background information
- references
- glossary of toxicological terms

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This page was last modified:
January 30, 2005


 

A Toxicology Primer for Student Inquiry:
Biological Smoke Detectors

by Charles Drewes

SAFETY

Obtain and study “Material Safety Data Sheets” (MODS) and/or International Chemical Safety Cards for all solvents and test chemicals that you will use in testing (Figs. 5 and 6).  MODS sheets and International Chemical Safety Cards are readily available from the University of Vermont which maintains a huge electronic data base relating to chemical safety.  The web address is:  http://hazard.com/msds/  

Learn and follow all safety measures for the laboratory facility in which you will be carrying out your study.  Learn and follow all written safety precautions for the chemicals you are using.  Handle all volatile or toxic materials in a fume hood.  Wear a lab coat, protective vinyl (or latex) gloves and use protective eyewear when opening or handling any chemical storage containers, stock solutions, pipettes, or exposure containers.       

Clearly label the contents and concentrations of all chemical solutions in containers.  Properly dispose of all used solutions, surplus solutions, or chemically-exposed materials such as pipette tips or filter paper.  Use absorbent towel to thoroughly remove any drips or spills of solutions to which humans may come in contact.  Thoroughly and carefully scrub and clean all glassware or plasticware that was exposed to chemicals.  Use ethanol and then water rinses to clean containers that held water-insoluble chemicals.  It is very important not to leave any chemical residues on glassware, thus the emphasis on careful cleaning. 

Finally, it is important to begin experiments without any traces of soap residue on glassware.  Soap residues are especially toxic to many aquatic invertebrates. 

Figure 5.  Sample MODS sheet for d-limonene.

Figure 6.  Sample International Chemical Safety card for d-limonene.


Next Section: exposure methods

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